Composite

Part:BBa_K4362082:Design

Designed by: Yun Wang   Group: iGEM22_Tianjin   (2022-09-11)


plasmid tightly regulated copy-control


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 729
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 683
    Illegal BglII site found at 2353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 729
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 729
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
    Illegal NgoMIV site found at 89
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 220
    Illegal NgoMIV site found at 1156
    Illegal AgeI site found at 1280
    Illegal AgeI site found at 2197
    Illegal AgeI site found at 3097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3951
    Illegal SapI site found at 2946


Design Notes

None.


Source

This part consists of several basic parts mentioned below.

References